16S-like rDNA sequence and phylogenetic position of the diplomonad Spironucleus muris (Lavier 1936)

Autor:	Manfred Berchtold, Jörg Reimann, Helmut König, Alfred Breunig, Jürgen Branke
Rok vydání:	1996
Předmět:	Genetics Phylogenetic tree biology Diplomonad Sequence analysis parasitic diseases Protozoa Diplomonadida Ribosomal RNA Flagellate biology.organism_classification Microbiology Spironucleus
Zdroj:	European Journal of Protistology. 32:227-233
ISSN:	0932-4739
DOI:	10.1016/s0932-4739(96)80022-8
Popis:	Summary The sequence of the small subunit (SSU) rDNA gene of the facultative pathogenic flagellate Spironucleus muris has been determined. This flagellate belongs to the class Diplomonadida. We will set out its phylogenetic position within the eukaryotic tree according to its morphological characteristics. Cells of Spironucleus muris were enriched from the digestive tract of immunodeficient mice and the DNA was extracted. After sequencing the SSU rDNA insert (1206 bp), we carried out a comparative sequence analysis with accessible SSU rRNA sequences from related eukaryotes. Escherichia coli and Methanospirilium hungatei were used as outgroups. Spironucleus muris shows strongest structural similarity (73.2%) to the parasitic flagellate Hexamita inflata (class Diplomonadida), which originated very early in the eukaryotic tree.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::1567016d3fe9bc28786a526f6710c728 https://doi.org/10.1016/s0932-4739(96)80022-8 Zobrazit plný text záznamu Full Text from ScienceDirect