A microfluidic device with integrated coaxial nanofibre membranes for optical determination of glucose
| Autor: | Jorge F. Fernández-Sánchez, Alberto Fernández-Gutiérrez, Adama M. Sesay, Antonio L. Medina-Castillo, Peter Panjan, Teresa Ramon-Marquez |
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| Rok vydání: | 2017 |
| Předmět: |
Materials science
Microfluidics chemistry.chemical_element Nanotechnology 02 engineering and technology 01 natural sciences Oxygen Adsorption Materials Chemistry Glucose oxidase Electrical and Electronic Engineering Instrumentation biology 010401 analytical chemistry Metals and Alloys 021001 nanoscience & nanotechnology Condensed Matter Physics 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Membrane Transducer chemistry Chemical engineering biology.protein Coaxial 0210 nano-technology Biosensor |
| Zdroj: | Sensors and Actuators B: Chemical. 250:156-161 |
| ISSN: | 0925-4005 |
| Popis: | In this work, a multifunctional material with a core–shell structure containing an inner optical oxygen transducer (PdTFPP) has been successfully used for the immobilization of glucose oxidase on its outer surface and the subsequent determination of glucose. The material was fabricated by co-electrospinning and immobilizing the enzyme by physical adsorption. The sensing mechanism is based on glucose oxidase oxidation of glucose that creates a localized decrease in the dissolved oxygen amount and consequently produces a measurable increase in the luminescence intensity of the inner oxygen transducer. The material was applied to detect glucose at room temperature, and exhibited a good luminescent response. Furthermore, this coaxial material was integrated into a microfluidic chip and its sensitivity to glucose was tested (LOD of 35 μM and LOQ of 105 μM), obtaining higher sensing properties than using the membrane alone under ambient conditions. This improvement in the sensing response can be explained by considering that the chip limits the oxygen transfer from the ambient air to the coaxial membrane, creating a more controlled environment in where to carry out the measurements. Therefore, the combination of this core–shell material with microfluidic devices could have great potential in the fabrication of oxygen dependent optical biosensors. |
| Databáze: | OpenAIRE |
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