Liquid–Liquid Microextraction of Tetracyclines from Biological Fluids for Their Subsequent Determination by High-Performance Liquid Chromatography with UV Detection

Autor:	K. D. Cherkashina, A. I. Sumina, Andrey Bulatov, K. S. Vakh
Rok vydání:	2020
Předmět:	Detection limit Aqueous solution Chromatography medicine.drug_class Chemistry Tetracycline 010401 analytical chemistry Tetracycline antibiotics Extraction (chemistry) Oxytetracycline 010402 general chemistry 01 natural sciences High-performance liquid chromatography 0104 chemical sciences Analytical Chemistry Blood serum medicine medicine.drug
Zdroj:	Journal of Analytical Chemistry. 75:1424-1429
ISSN:	1608-3199 1061-9348
Popis:	We proposed a liquid–liquid microextraction procedure for the isolation and preconcentration of tetracycline antibiotics from biological fluids. The procedure involves in situ formation of a micellar phase of the primary amine from an aqueous sample solution with the introduction of a polar organic solvent. Possibilities of the developed procedures are demonstrated on an example of the determination of tetracycline, oxytetracycline, and doxycycline in human plasma, blood serum, and urine by HPLC–UV. The limits of detection (3σ) for oxytetracycline and doxycycline were 0.03 mg/L, for tetracycline, it was 0.08 mg/L. The extraction time does not exceed 5 min.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::1562d996da17613209fa798369ba8ee6 https://doi.org/10.1134/s1061934820090075 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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