Circulating Tumor Cells: Markers and Methodologies for Enrichment and Detection
Autor: | José A. Lorente, Alba Rodríguez-Martínez, Inmaculada Robles-Fernandez, Jose Luis Garcia Puche, M.J. Álvarez Cubero, María José Serrano |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
business.industry Cancer Cancer detection medicine.disease Primary tumor 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Circulating tumor cell 030220 oncology & carcinogenesis Circulatory system Cancer research Medicine In patient Platelet business Whole blood |
Zdroj: | Methods in Molecular Biology ISBN: 9781493971435 |
Popis: | Cancer is a leading cause of disease worldwide; however, nowadays many points of its initiation processes are unknown. In this chapter, we are focusing on the role of liquid biopsies in cancer detection and progression. CTCs are one of the main components of liquid biopsies, they represent a subset of tumor cells that have acquired the ability to disseminate from the primary tumor and intravasate to the circulatory system. The greatest challenge in the detection of CTCs is their rarity in the blood. Human blood consists of white blood cells (5-10 × 106/mL), red blood cells (5-9 × 109/mL), and platelets (2.5-4 × 108/mL); very few CTCs will be present even in patients with known metastatic disease, with often less than one CTC per mL of blood. CTCs are found in frequencies on the order of 1-10 CTCs per mL of whole blood in patients with metastatic disease, and it is reduced in half for non-metastatic stages. Therefore, accurate methodologies for their capture and analysis are really important. The main aim of the present chapter is to describe different methodologies for CTCs capturing and analysis. |
Databáze: | OpenAIRE |
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