The use of single image random dot stereograms for presenting 3D microscopic confocal images

Autor:	Elizabeth J. Pettit, Maurice Bartlett Hallett
Rok vydání:	1997
Předmět:	Histology genetic structures business.industry Computer science Confocal Slicing Pathology and Forensic Medicine law.invention Optics Confocal microscopy law Random dot stereogram Grey level Computer vision Artificial intelligence Single image business
Zdroj:	Journal of Microscopy. 186:275-278
ISSN:	1365-2818 0022-2720
DOI:	10.1046/j.1365-2818.1997.2130769.x
Popis:	Presenting 3D images produced by confocal optical slicing techniques in a static and easily publishable form can be difficult. Here, we demonstrate the presentation of the data as a single image random dot stereogram (SIRD), which can be viewed as a 3D object by ‘defocusing’ the eyes. The production of the SIRD employs three steps: (i) acquisition of the optical slices using confocal techniques, (ii) allocation of a suitable grey level to the object in each slice to provide depth-encoding information for the final image and (iii) calculation of the SIRD from the composite depth-encoded image. The technique is demonstrated with a limited number of optical slices through an acridine-orange-stained neutrophil (diameter =10 μm), in order to show the relative positions of the nuclear lobes in the cell.
Databáze:	OpenAIRE
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