Determination of Ibuprofen in human plasma by high-performance liquid chromatography: validation and application in pharmacokinetic study
| Autor: | M Costa, E. Berno, Roberto Canaparo, G. P. Zara, Elisabetta Muntoni, Mario Eandi, C. Della Pepa |
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| Rok vydání: | 2000 |
| Předmět: |
Pharmacology
Detection limit Chromatography Chemistry Clinical Biochemistry Spectrofluorometer General Medicine Ibuprofen Biochemistry High-performance liquid chromatography Analytical Chemistry Pharmacokinetics Human plasma Drug Discovery Healthy volunteers medicine Enantiomer Molecular Biology medicine.drug |
| Zdroj: | Biomedical Chromatography. 14:219-226 |
| ISSN: | 1099-0801 0269-3879 |
| DOI: | 10.1002/1099-0801(200006)14:4<219::aid-bmc969>3.0.co;2-z |
| Popis: | A specific method for the simultaneous determination of S-(+)Ibuprofen and R-(-)Ibuprofen enantiomers in human plasma is described. Adopting a high-performance liquid chromatographic (HPLC) system with spectrofluorometer detector, the compounds were extracted from plasma in alcohol medium and were separated on C18 column, using a solution of acetonitrile-water-acetic acid-triethylamine as mobile phase. The limit of quantitation was 0.1 microg/mL for both compounds. The method was validated by intra-day assays at three concentration levels and was used in a kinetic study in healthy volunteers. During the study we carried out inter-day assays to confirm the feasibility of the method. |
| Databáze: | OpenAIRE |
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