Isolation and characterization of a plasmid fromTreponema denticola

Autor:	R. R. B. Russell, A. Ivic, J MacDougall, Charles W. Penn
Rok vydání:	1991
Předmět:	Plasmid preparation Treponema Treponema denticola Biology Molecular cloning biology.organism_classification Microbiology Molecular biology stomatognathic diseases Restriction map Plasmid Agarose gel electrophoresis Genetics Molecular Biology Southern blot
Zdroj:	FEMS Microbiology Letters. 78:189-194
ISSN:	1574-6968 0378-1097
Popis:	Agarose gel electrophoresis of whole genomic DNA of the oral spirochaete Treponema denticola has revealed a plasmid-like fraction. Purification and restriction enzyme analysis has confirmed the presence of a 2.6-kb circular plasmid, which has been mapped for restriction sites and cloned into the Escherichia coli plasmid pUC18. Southern blot analysis of genomic T. denticola DNA, using the plasmid as a probe, has shown that the plasmid is present only as an extra-chromosomal element. No plasmid-coded recombinant gene product from a PstI insert in pUC18 has been detected in host cells of E. coli by SDS-PAGE or immunoblotting with polyclonal immune rabbit serum to T. denticola. The discovery of this plasmid may provide a useful tool in the application of new molecular approaches in spirochaetal biology.
Databáze:	OpenAIRE
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