Identification of Human Cutaneous Basal Cell Carcinoma Cancer Stem Cells
| Autor: | Huw Morgan, Girish Khandubhai Patel, Carlotta Olivero |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
animal structures integumentary system Chemistry Proliferative capacity fungi Cell medicine.disease 03 medical and health sciences 030104 developmental biology medicine.anatomical_structure Cancer stem cell In vivo Gene expression medicine Cancer research Basal cell carcinoma Skin cancer skin and connective tissue diseases Gene |
| Zdroj: | Skin Stem Cells ISBN: 9781493988693 |
| DOI: | 10.1007/7651_2018_133 |
| Popis: | The cancer stem cell model states that a subset of tumor cells, called "cancer stem cells," can initiate and propagate tumor growth through self-renewal, high proliferative capacity, and their ability to recreate tumor heterogeneity. In basal cell carcinoma (BCC), we have shown that tumor cells that express the cell surface protein CD200 fulfill the cancer stem cell hypothesis. CD200+ CD45- BCC cells represent 0.05-3.96% of all BCC cells and reside in small clusters at the tumor periphery. Using a novel, reproducible in vivo xenograft growth assay, we determined that tumor-initiating cell (TIC) frequencies are approximately 1 per 1.5 million unsorted BCC cells. The CD200+ CD45- BCC subpopulation recreated BCC tumor growth in vivo with typical histological architecture and expression of sonic hedgehog-regulated genes. Reproducible in vivo BCC growth was achieved with as few as 10,000 CD200+ CD45- cells, representing ~1500-fold enrichment. The methods used to identify and purify CD200+ CD45- BCC cells, as well as characterize gene expression, are described herein. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|