| Popis: |
The present method includes spectrophotometric determination of niclosamide (NICS) via Schiff base reaction in an alcoholic medium (ethanol) after reducing the nitro group in NICS by added zinc powder in an acidic medium to produce a primary amine group which can be coupled with the salicylaldehyde (SSA) reagent to give a yellow solution whose absorption is followed at the wavelength of 405 nm. Beer's law limitation for the proposed method was in the range of concentrations of 4.0-30 μg. ml-1. The molar absorbance value was 8.701 x 104 l mol.-1 cm.-1 and Sandell's sensitivity value was 0.0376 μg. cm-2. The effect of the reaction components was studied separately by changing one of the components and keeping the other constant, and the optimal conditions were chosen for all these studied components. The proposed method has been successfully applied for the determination of NICS in a pharmaceutical formulation (Yumesan tablets). The recovery percentage of the medicinal compound under study (niclosamide) from the tablet pharmaceutical preparation (Yumesan) via applying the suggested method was not less than 96.36%, and the values of each of the low detection limit (LOD), the low quantitation limit (LOQ), the relative error percentage (RE%), and the relative standard deviation percentage (RSD%), their values were calculated as they are listed 0.9778 μg. ml-1, 3.259 μg. ml-1, -3.61% and not more than 3.67% respectively. |
