The effect of beta-adrenergic stimulation in the expression of the urokinase plasminogen activator receptor in bone marrow mesenchymal stem cells
| Autor: | Samira Mohammadzadeh, Elham Roshandel, Abbas Hajifathali, Maryam Nikoonezhad, Arsalan Jalili, Mohammad Hossein Kazemi, Ronak Nalini |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Urokinase Agonist medicine.diagnostic_test Chemistry medicine.drug_class Mesenchymal stem cell Flow cytometry Urokinase receptor 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure 030220 oncology & carcinogenesis Genetics medicine Cancer research Bone marrow Receptor Plasminogen activator medicine.drug |
| Zdroj: | Gene Reports. 22:101017 |
| ISSN: | 2452-0144 |
| DOI: | 10.1016/j.genrep.2021.101017 |
| Popis: | Background The migration of bone marrow mesenchymal stem cells (BMMSCs) from bone marrow (BM) to the damaged sites is the first step in regenerative medicine. BMMSCs express all urokinase system components include; urokinase plasminogen activator (uPA), uPA receptor (uPAR), and plasminogen activator inhibitor-1 (PAI-1) that are shown to affect BMMSCs migration. Also, it has been reported that the signaling of the β-adrenergic receptors (β-AdR) on BMMSCs increase their mobilization from bone marrow to the periphery. In this study, we surveyed the expression of β-AdRs in BMMSCs and the role of β-AdR stimulation using isoproterenol (β-Adrenergic agonist) in the expression of urokinase plasminogen activator receptor (uPAR) in BMMSCs. Methods We characterized BMMSCs isolated from bone marrow aspiration of two patients by their surface markers. The expression of β-AdRs, including β1-AdR, β2-AdR, and β3-AdR in MSCs were assayed using RT-PCR. BMMSCs were treated with isoproterenol, and uPAR mRNA expression was measured in three time-points (1, 3, and 5 h after adding isoproterenol) using qRT-PCR. Results The flow cytometry analysis result proved the specific characters of MSCs. The expression of uPAR increased in all three time-points of isoproterenol treatment, but a significant increase in uPAR mRNA was seen in MSCs treated 5 h with isoproterenol, compared to the untreated MSCs and also MSCs treated less than 5 h (1 and 3 h). Conclusion According to the results, beta-adrenergic signaling can increase the expression of uPAR in MSCs, which might facilitate their mobilization into the peripheral blood. |
| Databáze: | OpenAIRE |
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