| Popis: |
The aim of this study is focused in the evaluation of the genetic diversity of C. violaceum isolated from Brazilian ecosystems. Strains from Malaysia, Amazonas, Pernambuco, Ceara were analysed by the 16S rRNA gene (amplified 16S ribosomal DNA restriction analysis) to define their phylogenetic positions. All strains were grown overnight in LB medium at 30°C at 150 rpm, and cooled on ice, and the DNA was amplified in a thermocycler. The primers used were fD1 (AGAGTTTGATCCTGGCTC AG) and rD1 (AAGGAGGTGATCCAGCC) complementary to the ends of the 16S rDNA. The data obtained herein demonstrated that this method allowed grouping the C. violaceum isolates according to different Brazil States. However, the distances genetics between among all the isolated ones studied in this work demonstrated low variability but that the use of 16S rDNA gene tree revealed the good correlation between phylogenetic clustering and geographic origin. |
