Sialic acid binding lectins from leaf of mulberry (Morus alba)
| Autor: | Wayakorn Ngamjunyaporn, Sunanta Ratanapo, Montri Chulavatnatol |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
biology
Size-exclusion chromatography Lectin Plant Science General Medicine Sialic acid binding Moraceae biology.organism_classification Sialic acid chemistry.chemical_compound chemistry Affinity chromatography Biochemistry Sephadex hemic and lymphatic diseases Genetics biology.protein neoplasms Agronomy and Crop Science Ammonium sulfate precipitation |
| Zdroj: | Plant Science. 139:141-148 |
| ISSN: | 0168-9452 |
| DOI: | 10.1016/s0168-9452(98)00185-x |
| Popis: | Two new lectins, MLL 1 and MLL 2, were purified from the leaves of mulberry ( Morus alba ) by a procedure consisting of ammonium sulfate precipitation, affinity chromatography on a N -acetylgalactosamine-agarose column, gel filtration on a Sephacryl S-200 column, anion-exchange chromatography using a DEAE-Sephacel column and gel filtration on a Sephadex G-75 column. Both MLL 1 and MLL 2 were found to be glycoproteins with neutral sugar contents of 8.8 and 40%, respectively. They showed the same native molecular weight of 51 000. Each consisted of subunit with molecular weight of 16 500. They were highly specific for N -glycolylneuraminic acid. |
| Databáze: | OpenAIRE |
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