Extraction of lignans from Phyllanthus amarus Schum. & Thonn using pressurized liquids and low pressure methods
| Autor: | Rúbner Gonçalves Pereira, Vera Lúcia Garcia, Julian Martínez, Marili V.N. Rodrigues |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Lignan Chromatography 010405 organic chemistry Extraction (chemistry) Filtration and Separation Mass spectrometry 01 natural sciences High-performance liquid chromatography 0104 chemical sciences Analytical Chemistry Solvent 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Yield (chemistry) Gas chromatography Chemical composition |
| Zdroj: | Separation and Purification Technology. 158:204-211 |
| ISSN: | 1383-5866 |
| Popis: | Extracts rich in lignans were obtained from Phyllanthus amarus by Pressurized Liquid Extraction (PLE). The influences of temperature and solvent type on the extraction yield and chemical composition of the extracts were evaluated. Temperature was evaluated from 35 °C to 80 °C and the used solvents were: water, ethanol and a mixture of ethanol + water (50% v/v). All extractions were performed at the pressure of 10 MPa. A low pressure extraction method (LPE) using the Ultra-Turrax disperser and the same solvents, at room temperature and atmospheric pressure, was performed to compare the results with those of PLE. The extracts were analyzed by Gas Chromatography coupled to Mass Spectrometry (GC–MS) and seven lignans were identified: 5-demethoxy-niranthin, phyllanthin, filtetralin, 5-demethoxy-nirtetralin, nirtetralin, hipophyllanthin and niranthin. High Performance Liquid Chromatography (HPLC) was used to quantify the most bioactive lignans, phyllanthin and niranthin, in the extracts. In the PLE method, the effect of temperature was negligible in the studied range for the recovery of these lignans. The binary mixture ethanol + water (50% v/v) was the best solvent for the recovery of high amounts of both lignans, independently of the extraction method. However, PLE with 100% ethanol provided the most concentrated extracts in both lignans. PLE achieved higher global extraction yield than in LPE, but lower lignan recoveries. The contents of the major lignans extracted by each method varied significantly, suggesting that phyllanthin and niranthin are located in different parts of the plant tissue. The selection of the best method to extract lignans form P. amarus depends on the operation costs. |
| Databáze: | OpenAIRE |
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