On Chip Monitoring of Tight Junctions Formed on an Epithelial Monolayer and the Effect of Extracellular Calcium Ion Removal from Apical and Basal Sides
| Autor: | Yang Liu, Yuji Takata, Kiyotaka Tsuji, Ryuji Yokokawa, Kaori Naganuma, Ryohei Ueno, Ramin Banan Sadeghian |
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| Rok vydání: | 2020 |
| Předmět: |
Materials science
Tight junction 010401 analytical chemistry Epithelial monolayer chemistry.chemical_element 02 engineering and technology Calcium 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences Basal (phylogenetics) medicine.anatomical_structure chemistry Cell polarity Biophysics Extracellular medicine Proximal tubule 0210 nano-technology Barrier function |
| Zdroj: | 2020 IEEE 33rd International Conference on Micro Electro Mechanical Systems (MEMS). |
| Popis: | We designed a microfluidic device to measure the trans-epithelial electrical resistance (TEER) and demonstrated that the tight junctions (TJs) of human renal proximal tubule epithelial cells (RPTECs) respond differently to the presence or absence of extracellular Ca2+ at the apical and basal sides. Once Ca2+ was removed from the basal side, the structure of TJs collapsed rapidly, whereas when the ion was removed from the apical side the collapse was gradual. After the removal of Ca2+, when it was resupplied to the basal side, the TJs recovered rapidly, whereas when the ion was replenished at the apical side, the recovery was gradual. We detected a subtle difference in the appearance of cell polarity. That indicates that our developed device is capable to accurately measure the TEER. The results of the deference in the response of TJs for extracellular Ca2+ may lead to the elucidation of the operating principle of the barrier function which tight TJs form. |
| Databáze: | OpenAIRE |
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