A Homeodomain Protein Binding Element in the Bone Sialoprotein Promoter is Critical for Tissue-Specific Expression in Bone
| Autor: | Peedikayil E. Thomas, Rajaram Gopalakrishnan, Dian Wang, Renny T. Franceschi, M. Douglas Benson |
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| Rok vydání: | 2003 |
| Předmět: |
musculoskeletal diseases
Bone sialoprotein Genetically modified mouse biology Calvaria Cell Biology Plasma protein binding Biochemistry Molecular biology medicine.anatomical_structure stomatognathic system Rheumatology In vivo medicine biology.protein Homeobox Orthopedics and Sports Medicine Luciferase Molecular Biology Gene |
| Zdroj: | Connective Tissue Research. 44:154-160 |
| ISSN: | 1607-8438 0300-8207 |
| DOI: | 10.1080/03008200390152250 |
| Popis: | Bone sialoprotein (BSP) expression is restricted to cells associated with the mineralization of bones and teeth. We previously identified a homeodomain binding element in a 2.5 kb fragment of the murine Bsp promoter that is required for osteoblast-selective expression in cell culture. To examine the role of this element (called OSHE1; osteoblast-specific homeodomain element 1) in the tissue-specific expression of Bsp in vivo, we generated transgenic mice using the wild-type 2472 bp promoter or the same promoter containing a 2 bp mutation in OSHE1. Promoter constructs driving both luciferase and lacZ reporter genes were microinjected into fertilized eggs from (C57BL/6 X SJL)F1 mice. Four lines containing the wild-type promoter and 5 lines containing the mutated promoter were established, and the tissue specificity of g -galactosidase staining and luciferase expression was examined. g -gal staining was observed in osteoblasts of calvaria and trabecular regions of tibia and femur in 12-day-old mice w... |
| Databáze: | OpenAIRE |
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