BIOREMEDIATION OF SOME AGRICULTURAL WASTES BY BACILLUS THURINGIENSIS FOR METALLOPROTEASE PRODUCTION
| Autor: | Ayman S. Eldourghamy, Nashwa M. H. Rizk, Eman G. Elgamal |
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| Rok vydání: | 2017 |
| Předmět: |
chemistry.chemical_classification
Bran biology Biodegradation biology.organism_classification Enzyme assay chemistry.chemical_compound Enzyme Bioremediation chemistry Sephadex Bacillus thuringiensis biology.protein General Earth and Planetary Sciences Food science Sulfate General Environmental Science |
| Zdroj: | Research Journal of Applied Biotechnology. 3:11-22 |
| ISSN: | 2356-9433 |
| Popis: | Agricultural wastes are very rich in nutrients that can be used as complete balanced microbiological media for growing microbes in order to produce valuable commercial products. In this study, a biodegradation process of wheat bran and rice bran wastes was carried out to produce metalloprotease enzyme from B. thuringiensis 1257strain which is considered to be a hyper producer for that enzyme. Metalloprotease was partially purified by ammonium sulfate followed by application on Sephadex G-75 column. Gel filtration step resulted in more than 40 times fold purification of the purified enzyme. The enzyme activity was inhibited by EDTA (almost 80%) at 15 mM concentration. These agro-industrial wastes were used as substrates for economic production of the enzyme from B. thuringiensis compared with some bacterial isolates collected from different sites in Kafr El-Sheikh and Menofiya governorate, Egypt. Optimum temperature for enzyme activity was 40 °C. It also exhibited a broad pH activity range (6-9) with an optimum pH of 8. |
| Databáze: | OpenAIRE |
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