Extraction and fractionation of polyunsaturated fatty acids fromMortierellasp. using supercritical fluid: experimental and kinetic studies

Autor: Ajay K. Dalai, Janusz A. Kozinski, Ashwini Tilay, Uday S. Annapure, Ramin Azargohar
Rok vydání: 2014
Předmět:
Zdroj: Asia-Pacific Journal of Chemical Engineering.
ISSN: 1932-2135
DOI: 10.1002/apj.1779
Popis: The growing interest in various applications of polyunsaturated fatty acids daily dietary requirements and demand has directed much attention on the industrial production of bio-oil from plant, animal, fungi, or algae sources. This work signifies that the extraction of polyunsaturated fatty acids from fungal biomass of Mortierella sp. 1458 using supercritical CO2 (SC-CO2) with subsequent fractionation of various fatty acids present in biomass using co-solvents was investigated. The use of co-solvent in supercritical fluid extraction has provided the green system as a substitute for classical solvent extraction at industrial scale. The optimized extraction temperature (62.3 °C), pressure (298.5 bar), and time (88.6 min) on SC-CO2 extraction conditions gave maximum recovery up to 66.35% of bio-oil from dried biomass. Further co-solvent addition to SC-CO2 has improved % recovery up to 97.88%. The bio-oil obtained by this method was examined for use by characterization with value added chemicals such as omega-3, omega-6, and omega-9 fatty acids. Peleg's equation was used to study the bio-oil extraction kinetics. The equilibrium yield as well as the time required to reach half of equilibrium yield were also calculated. It showed that for an effective extraction process with high yield, CO2 density larger than 700 kg/m3 is needed. © 2014 Curtin University of Technology and John Wiley & Sons, Ltd.
Databáze: OpenAIRE
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