49 USE OF DILUTION OF CRYOPRESERVED SEMEN IN FIXED-TIME ARTIFICIAL INSEMINATION OF MANGALARGA MARCHADOR MARES
| Autor: | José Camisão de Souza, A. C. Rezende, A. C. F. Farinasso, M. B. R. Nogueira, T. L. C. Pinto |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
medicine.medical_specialty
medicine.medical_treatment media_common.quotation_subject Semen Reproductive technology law.invention Andrology Endocrinology law Internal medicine Genetics medicine Molecular Biology Ovulation media_common Pregnancy biology Artificial insemination Extender Mangalarga medicine.disease biology.organism_classification Pregnancy rate Reproductive Medicine Animal Science and Zoology Developmental Biology Biotechnology |
| Zdroj: | Reproduction, Fertility and Development. 24:136 |
| ISSN: | 1031-3613 |
| Popis: | Equine AI with frozen semen has become more frequent in reproduction centers, but osmotic shock during thawing is still a major problem as it impairs gamete viability and fertility. To overcome this, multi ultrasonographic (US) exams must be performed to ensure AI at the closest time to ovulation possible. The objective was to evaluate, in vivo, the effects of dilution of the cryoprotectant dimethylphormamide on the success of a fixed timed (20 h post-ovulation) AI protocol, in an attempt to reduce osmotic shock and the intense labour usually required during equine AI with frozen semen. Thirty-five Mangalarga Marchador mares were used in this trial. When dominant follicles reached at least 35 mm in diameter, ovulation was induced with 6 mg of equine gonadotrophin extract (EGE) intravenously. Artificial insemination was performed 20 h after ovulation according to 1 of the following protocols: no dilution (n = 14): three 0.5-mL straws were thawed and the semen deposited by a flexible rod at the uterine tip in the ipsilateral horn where the pre-ovulatory follicle was detected; or dilution (n = 21): three 0.5-mL straws were thawed as described and centrifuged after addition of 1.5 mL of BotuSêmen® extender (1:1 dilution). Pregnancy rates were evaluated between treatments, age and presence of intrauterine liquid (IUL) classes and period between ovulation and AI. Data were analysed as a binomial distribution with the GENMOD procedure (SAS, Cary, NC, USA). Pregnancy rate was higher (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|