Propionibacterium acnes: interaction with complement and development of an enzyme-linked immunoassay for the detection of antibody

Autor: Craig G. Burkhart, Msph, John Cantrill, Cynthia L. Butcher, Paul F. Lehmann
Rok vydání: 1999
Předmět:
Zdroj: International Journal of Dermatology. 38:200-203
ISSN: 1365-4632
0011-9059
DOI: 10.1046/j.1365-4362.1999.00674.x
Popis: Objective To characterize the immune response to Propionibacterium acnes in acne patients. Design Comparison of serologic responses in acne and normal patients using counterimmunoelectrophoresis for antibody and an enzyme-linked immunosorbent assay (ELISA) to detect immunoglobulin G (IgG) antibody. Setting The serum of acne and nonacne patients from the Dermatology Clinic at the Medical College of Ohio was utilized for analysis. Results Using counterimmunoelectrophoresis, antibody was detected in 13 of 20 acne patients. The antigen was detectable as an anion in the barbital buffer at pH 8.2, strongly suggesting a carbohydrate component. By ELISA, the antibody proved to be IgG, and the bacteria and its water-soluble fractions were capable of fixing complement. Conclusions The primary instigator of inflammation in acne vulgaris is an immunologic reaction to extracellular products of P. acnes. The immunologic response involves both humoral and cell-mediated pathways. The antibodies to P. acnes have not been characterized fully, although they are largely of the IgG class. We have further characterized the dominant antigen to have a carbohydrate component.
Databáze: OpenAIRE
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