Einfache Temperatursprung-Methode im Sekundenbis Stundenbereich und die reversible Denaturierung von Chymotrypsin

Autor:	F. M. Pohl
Rok vydání:	1968
Předmět:	Reaction rate constant Chemistry law Kinetics Microcell Physical chemistry Molecule Thermodynamics Denaturation (biochemistry) Optical density Biochemistry Thermostat law.invention
Zdroj:	European Journal of Biochemistry. 4:373-377
ISSN:	1432-1033 0014-2956
Popis:	A simple method for measuring the changes of the optical density with time, after heating or cooling a solution within seconds, is described. The temperature-changes in the solution are achieved by switching the circulating fluids of two thermostats alternating through a microcell. Possible applications of this method are discussed. The kinetics of the reversible denaturation of α-Chymotrypsin in the pH-range of 2.0–2.5 were measured. These measurements are in agreement with a transition between two different states of the protein molecule and allow the evaluation of the rate constants. The temperature dependence of these rate-constants is presented and discussed by a qualitative picture of the denaturation process.
Databáze:	OpenAIRE
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