Cell cycle-dependent suppressive effect of histone H1 on mitosis-specific H3 phosphorylation
| Autor: | K Ajiro, K Shibata |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
inorganic chemicals
biology macromolecular substances Cell Biology Cell cycle environment and public health Biochemistry Molecular biology Cell biology enzymes and coenzymes (carbohydrates) Histone H3 Histone Histone H1 Histone H2A biology.protein bacteria Phosphorylation Protein phosphorylation Molecular Biology Mitosis |
| Zdroj: | Journal of Biological Chemistry. 268:18431-18434 |
| ISSN: | 0021-9258 |
| DOI: | 10.1016/s0021-9258(17)46642-7 |
| Popis: | To analyze the mechanism by which histone H3 phosphorylation occurs specifically during mitosis, the effect of H1 on mitosis-specific H3 phosphorylation (Ser-10) was investigated in nucleosomes. H1 interaction with H1-depleted nucleosomes suppressed H3 phosphorylation including Ser-10 by approximately 50%. However, H1 interaction with DNA-free histone octamers failed to suppress H3 phosphorylation. The extent of suppression of H3 phosphorylation in nucleosomes with H1 prepared from synchronized HeLa cells was cell cycle-dependent. Binding with a highly phosphorylated mitotic H1 produced the least suppression of H3 phosphorylation, whereas binding with a lower H1 phosphorylation from G1 phase resulted in the greatest suppression. The results suggest that 1) mitotic H3 phosphorylation is suppressed with a lower level of H1 phosphorylation during interphase and 2) highly phosphorylated H1 during mitosis partially releases the suppression of mitotic H3 phosphorylation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|