Utility of Calretinin Stain in Work-up of Inadequate Biopies in Patient’s with Hirschsprung’s Disease
| Autor: | Nagi Ah, Sikandar M, Naseem N |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
medicine.medical_specialty
medicine.diagnostic_test business.industry General surgery medicine.disease Stain Work-up Surgery 03 medical and health sciences 0302 clinical medicine 030220 oncology & carcinogenesis Biopsy medicine Histopathology In patient 030212 general & internal medicine Calretinin business Hirschsprung's disease Biomedical sciences |
| Zdroj: | Journal of Gastrointestinal & Digestive System. |
| ISSN: | 2161-069X |
| DOI: | 10.4172/2161-069x.1000515 |
| Popis: | Background and Objective: A thorough study was designed with an objective of observing utility of calretinin as immune-histochemical marker for aganglionosis and for detection of ganglion cells in the inadequate biopies of affected areas for more accurate and better diagnosis of the disease. Study Design: It was observational, descriptive study. Setting: It was carried out at the Department of Morbid Anatomy and Histopathology in University of Health Sciences (UHS) Lahore. Period: The study commenced in March 2016 after approval of the synopsis by the Advance Studies and Research Board of UHS and was successfully completed in December 2016. Methodology: Biopsy specimens of colon which were considered for the study were collected from 73 patients from Mayo Hospital and Jinnah Hospital, Lahore with established histopathologically diagnosed HSCR on H&E staining. Results: The mean age was 12.52 ± 9.21 months. On the basis of Calretinin staining Ganglion cells were present in 42/73 (57.53%) and absent in 31/73 (42.47%) respectively. Conclusion: It was consummated that Calretinin provides a reliable and very cost effective adjunctive test to be used routinely with H&E in diagnosing HSCR and consequently waiving off the need for unnecessary surgeries and repeated biopsies. |
| Databáze: | OpenAIRE |
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