| Popis: |
Serological studies were carried out with isolated and reference strains. Extract was prepared according to the method of Rantz and Randall, and antiserum was prepared by immunizing rabbit with formalin-killed organisms.Precipitin reaction (ring prove) was observated for 2 hours at room temperature.The antigen titer of the strain belonging to S. sanguis type I is almost constant, 1: 128-1: 256, while the antibody titer is not constant. In the serological experiment, antiserum with as high antibody titer as possible was used.Among the extracts in twofold dilution made of streptococci isolated from the dental plaque, precipitin reaction were carried out only in 39 strains reacting intensely with S. sanguis type I antiserum alone, and 35 strains of these were identified as S. sanguis type I. The biological properties of these strains resemble those of S. sanguis type I.Strains Challis and M-5 were found to be serologically most similar to S. sanguis type I-II ATCC 10558. Strain 8144, 12396, F 90 A and Wicky in the group H streptococcus gave different results. |
