| Autor: | Cody C. Cain, R. A. Walker, David E. Saslowsky, Brenda W. Shirley |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
chemistry.chemical_classification
Chalcone isomerase Chalcone synthase biology food and beverages Plant Science General Medicine biology.organism_classification medicine.disease_cause Molecular biology Fusion protein Enzyme Flavonoid biosynthesis Biochemistry chemistry Arabidopsis Genetics biology.protein medicine Arabidopsis thaliana Agronomy and Crop Science Escherichia coli |
| Zdroj: | Plant Molecular Biology. 35:377-381 |
| ISSN: | 0167-4412 |
| DOI: | 10.1023/a:1005846620791 |
| Popis: | Antibodies have been developed against the first two enzymes of flavonoid biosynthesis in Arabidopsis thaliana. Chalcone synthase (CHS) and chalcone isomerase (CHI) were overexpressed and purified from Escherichia coli as fusion proteins with glutathione S-transferase from Schistosoma japonicum. The recombinant proteins were then used to immunize chickens and the resulting IgY fraction was purified from egg yolks. Immunoblots of crude protein extracts from Arabidopsis seedlings carrying wild-type and null alleles for CHS and CHI showed that the resulting antibody preparations provide useful tools for characterizing expression of the flavonoid pathway at the protein level. An initial analysis of expression patterns in seedlings shows that CHS and CHI proteins are present at high levels during a brief period of early seedling germination that just precedes the transient accumulation of flavonoid end-products. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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