In vitro cell behavior of osteoblasts on Pyrost bone substitute

Autor: Jui-Sheng Sun, Yi Shiong Hang, Yang Hwei Tsuang, Hwa-Chang Liu, Feng-Huei Lin
Rok vydání: 1997
Předmět:
Zdroj: The Anatomical Record. 247:164-169
ISSN: 1097-0185
0003-276X
DOI: 10.1002/(sici)1097-0185(199702)247:2<164::aid-ar2>3.0.co;2-y
Popis: BACKGROUND Pyrost bone substitute has been shown to be a promising orthopedic biomaterial. However, little is known about mechanisms that are responsible for the genesis and development of the bond between bone and the Pyrost bone substitute. The purpose of this study is to elucidate the in vitro cell behavior of osteoblasts on Pyrost bone substitute. METHODS By using primary culture of rat osteoblasts, the changes in cell morphology during adhesion and flattening onto the surface of Pyrost bone substitute were studied in vitro. At 1 hour, at 3 hours, and at days 1, 3, and 7 after layering, the cell behavior was observed with scanning electron microscope. RESULTS The processes of trypsinized osteoblast adhesion and spreading on Pyrost bone substitute consisted of 1) contact of rounded osteoblasts with the Pyrost substrate; 2) attachment of osteoblasts at point of contact; 3) centrifugal growth of filopodia; 4) flattening and spreading of the osteoblasts on the Pyrost substrate; 5) division and growth of osteoblasts; and 6) suspension of the osteoblasts across the pores by their processes. CONCLUSIONS These results demonstrated that Pyrost can form a physico-chemical bond with osteoblasts. The Pyrost bone substitute not only supports osteoblasts attachment but also allows proliferation of the osteoblasts.
Databáze: OpenAIRE
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