A new mitochondria-targeting fluorescent probe for ratiometric detection of H2O2 in live cells
| Autor: | Nengqin Jia, Chusen Huang, Yang Wang, Chengcheng Wang, Guanyang Wang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Detection limit
Fluorescence-lifetime imaging microscopy Fluorophore 010401 analytical chemistry Quantum yield 02 engineering and technology 021001 nanoscience & nanotechnology 01 natural sciences Biochemistry Fluorescence 0104 chemical sciences Analytical Chemistry law.invention chemistry.chemical_compound chemistry Live cell imaging Confocal microscopy law Biophysics Environmental Chemistry Mitochondrion localization 0210 nano-technology Spectroscopy |
| Zdroj: | Analytica Chimica Acta. 1097:230-237 |
| ISSN: | 0003-2670 |
| Popis: | With this research we presented a ratiometric and mitochondria-target fluorescent probe (Mito-HT) for detection of H2O2 both in vitro and in live cells. Mito-HT was constructed by direct conjugation of aryl boronate to fluorophore with three synthetic steps. The borate group is cleaved from Mito-HT in the presence of H2O2, resulting in the exposure of the hydroxyl group of the electron donating group. Then the ICT mechanism was turned on, and the fluorescence emission of Mito-HT at 493 nm was red-shifted to 562 nm, thereby achieving radiometric detection of H2O2. Mito-HT exhibited a highly selectivity towards H2O2, and this interaction can be completed within 40 min. Mito-HT could be used for quantitative detection of H2O2 (0–200 μM) through ratiometric fluorescence signal readout. And limit of detection (LOD) is approximately 0.33 μM. The relatively high stability and medium fluorescence quantum yield of Mito-HT (0.39) and Mito-HT-OH (0.43) enable clear mitochondria localization and dual-channel fluorescence imaging of H2O2 in live cells with confocal microscopy. |
| Databáze: | OpenAIRE |
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