Rubisco Quantitation in Leaves of Different Barley Varieties by Enzyme-Linked Immunosorbent Assay

Autor:	Metodi V. Metodiev, K. Demirevska-Kepova
Rok vydání:	1992
Předmět:	inorganic chemicals chemistry.chemical_classification Oxygenase Physiology fungi Fresh weight RuBisCO food and beverages Plant Science Biology Pyruvate carboxylase Enzyme chemistry Biochemistry biology.protein Poaceae Hordeum vulgare Cultivar
Zdroj:	Journal of Experimental Botany. 43:155-158
ISSN:	1460-2431 0022-0957
DOI:	10.1093/jxb/43.2.155
Popis:	An enzyme-linked immunosorbent assay (ELISA) for the quantitation of Rubisco was developed. The amount of Rubisco in the primary leaves of ten different barley varieties was determined. It varied from 15 to 50 per cent of the total soluble protein and from 2-5 to 10 mg g1 fresh weight. The carboxylase and oxygenase activities of the extracts were consistent with the results of the immunoenzymatic assay. The variability of the Rubisco content among varieties suggests genetic determination.
Databáze:	OpenAIRE
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