Monocyte-Macrophage Mediated Suppression of Erythropoiesis in Renal Anemia
| Autor: | Silvia Carozzi, Silvano Lamperi |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
medicine.medical_specialty
Kidney business.industry medicine.medical_treatment Monocyte Continuous ambulatory peritoneal dialysis Interleukin Peripheral blood mononuclear cell medicine.anatomical_structure Endocrinology hemic and lymphatic diseases Internal medicine medicine Erythropoiesis Prostaglandin E2 business circulatory and respiratory physiology Prostaglandin E medicine.drug |
| Zdroj: | Molecular and Cellular Aspects of Erythropoietin and Erythropoiesis ISBN: 9783642726545 |
| DOI: | 10.1007/978-3-642-72652-1_21 |
| Popis: | To investigate monocyte-macrophage secretions in the pathogenesis of renal anemia, we studied 12 uremic patients and 12 normal subjects with respect to: 1. in vitro proliferation of erythroid progenitors (BFU-e) from uremic and normal peripheral blood whole mononuclear cells (WMNC); 2. effects of monocyte depletion of uremic or normal WMNC on the proliferation of BFU-e; 3. effects of adding uremic or normal peritoneal macrophages (PM) to peripheral blood normal non-adherent mononuclear cells (NAMNC); 4. Interleukin-1 (IL-1) and prostaglandin E2 (PGE2) concentrations in supernatants of uremic or normal PM. Results: 1. BFU-e growth from uremic WMNC is lower than from normal WMNC; 2. removal of monocytes induces an increase in BFU-e development in BFU-e in uremic patients, but a decrease in normal subjects; 3. addition of uremic PM to NAMNC causes a decrease in BFU-e colony formation compared to values obtained by incubating normal PM; 4. PGE2 release in supernatants of uremic PM is higher than in supernatants of normal PM; 5. IL-1 activity is lower from uremic PM than from normal PM. Conclusions: we found uremic monocyte-macrophages have a suppressive effect on BFU-e development in vitro which can be mediated by abnormal release of secretory products. |
| Databáze: | OpenAIRE |
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