Klasyfikacja molekularna gruczolaków przysadki: w poszukiwaniu kryteriów przydatnych do badań wysokoprzepustowych
| Autor: | Adam Rudnik, Michał Jarząb, Jolanta Krajewska, Piotr Bazowski, Jadwiga Żebracka-Gala, Kornelia Hasse-Lazar, Dawid Larysz, Barbara Jarząb |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
endocrine system medicine.medical_specialty endocrine system diseases Microarray Adenoma Endocrinology Diabetes and Metabolism Context (language use) Pituitary neoplasm Biology medicine.disease FSHB 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Endocrinology Real-time polymerase chain reaction Pituitary adenoma Internal medicine Gene expression medicine hormones hormone substitutes and hormone antagonists 030217 neurology & neurosurgery |
| Zdroj: | Endokrynologia Polska. 67:148-156 |
| ISSN: | 2299-8306 0423-104X |
| Popis: | Introduction: The mechanism of pathogenesis of pituitary adenomas is still unknown, and it shows differences in pituitary cells of different origin. The aim of our study was to analyse the gene expression profile of pituitary hormones and their precursor genes: PRL, GH, POMC, TSHb, LHb, FSHb, and CGA by QPCR in particular types of pituitary adenomas, and to evaluate the results in the context of sample selection for microarray studies. Material and methods: Analysis of the gene expression profile was performed in 84 samples of pituitary adenomas, by real-time quantitative PCR (QPCR). Results: As expected, expression of GH gene was significantly higher in somatotropinomas than in prolactinomas (p < 0.05). For POMC gene we noticed lower expression in all pituitary adenomas, except adrenocorticotropinomas (p < 0.05). In the case of PRL gene, the highest expression was observed; PRL+ adenomas were in third place. LHb and FSHb genes showed the highest expression, respectively, in LH-producing and FSH-producing pituitary adenomas; however, our analysis did not show statistically significant differences between LH-producing and FSH-producing adenomas. Conclusions: Our study showed that GH is a characteristic gene for somatotropinomas. We drew a similar conclusion for POMC gene and adrenocorticotropinomas. However, the results that we obtained for PRL, TSHb, LHb, FSHb, and CGA genes indicate that evaluation of gene expression is not sufficient for classification of particular subtypes of pituitary adenomas. (Endokrynol Pol 2016; 67 (2): 148–156) |
| Databáze: | OpenAIRE |
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