Measurement of excited singlet oxygen molecule in a vacuum sterilization system, using electric spin resonance (ESR) with a water-soluble polymer thin film
| Autor: | Satoru Iwamori, Kiyoshi Yoshino |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
010302 applied physics
Chemistry General Chemical Engineering General Physics and Astronomy chemistry.chemical_element 02 engineering and technology General Chemistry Sterilization (microbiology) 021001 nanoscience & nanotechnology Photochemistry 01 natural sciences Oxygen law.invention Magazine law Excited state Reagent 0103 physical sciences Molecule Thin film 0210 nano-technology Electron paramagnetic resonance |
| Zdroj: | Journal of Photochemistry and Photobiology A: Chemistry. 328:148-153 |
| ISSN: | 1010-6030 |
| Popis: | An advanced sterilization system employing active oxygen species (AOS) was investigated. We revealed in a previous paper that, by using active oxygen species generated from oxygen gas by ultraviolet irradiation in the chamber of the sterilization system, the survival curves of Geobacillus stearothermophilus spores (10 6 CFU), a biological indicator (BI), showed exponential reduction, and that this sterilization was attributable to the UV-generated AOS. However, we had little knowledge of what specific types of AOS were primarily responsible for the sterilization effects. Therefore, in this study we investigated the principal sterilization contributors among the AOS generated by ultraviolet irradiation, using the electron spin resonance (ESR) technique, with 2,2,6,6-tetramethyl-4-piperidinol (TEMP) as a spin-label reagent for the excited state of atomic oxygen molecules ( 1 O 2 ), and compared the respective sterilization effects of AOS and oxygen plasma system. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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