Distribution and Heterogeneity of Heterochromatin in the European Huchen (Hucho hucho Linnaeus, 1758) (Salmonidae)
| Autor: | Jankun Malgorzata, Ocalewicz Konrad, Fopp-Bayat Dorota, Liszewski Tomasz, Kucinski Marcin, Furgala-Selezniow Grazyna |
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| Rok vydání: | 2014 |
| Předmět: |
Genetics
medicine.medical_specialty Heterochromatin Cytogenetics Karyotype General Medicine Biology biology.organism_classification General Biochemistry Genetics and Molecular Biology Staining Endonuclease Restriction enzyme chemistry.chemical_compound chemistry Hucho hucho medicine biology.protein Chromomycin A3 |
| Zdroj: | Folia Biologica. 62:81-89 |
| ISSN: | 1734-9168 0015-5497 |
| DOI: | 10.3409/fb62_2.81 |
| Popis: | The chromosomal characteristics, locations and variations of the heterochromatin were studied in the European huchen (Hucho hucho, Linnaeus, 1758) karyotype using conventional C- banding, endonuclease digestion banding, silver nitrate (AgNO3), chromomycin A3 (CMA3) and DAPI staining techniques. The karyotype consists of 82 chromosomes: 13 pairs of metacentric chromosomes, 2 pairs of submetacentric chromosomes and 26 pairs of subtelo-acrocentric chromosomes (NF = 112). Original data on the chromosomal distribution of segments resistant to Alu I, Dde I and Mbo I restriction endonucleases and identification of the C-banded heterochromatin presented here have been used to characterize the huchen karyotype. On the basis of the banding patterns provided in the course of restriction enzyme digestion, AgNO3/CMA3 staining and C-banding we distinguished twelve types of heterochromatin grouped in four areas of the European huchen chromosomes. One pair of NOR-bearing chromosomes was found to be polymorphic in size and displayed two distinct forms. A combination of various staining techniques provided original data concerning heterochromatin distribution and heterogeneity in the European huchen which enabled better karyotypic definition of this fish species. |
| Databáze: | OpenAIRE |
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