Development of ultraviolet-polymerizable enzyme pastes: bioprocess applications of screen-printed L-lactate sensors

Autor:	Ursula Bilitewski, Meike Genrich, Wendy Collier, Ingrid Rohm
Rok vydání:	1996
Předmět:	Detection limit Chromatography Immobilized enzyme Chemistry Analytical chemistry Enzyme electrode Standard solution Biochemistry Amperometry Analytical Chemistry Linear range Electrochemistry Environmental Chemistry Bioprocess Biosensor Spectroscopy
Zdroj:	The Analyst. 121:877
ISSN:	1364-5528 0003-2654
DOI:	10.1039/an9962100877
Popis:	The development of an amperometric L-lactate-specific enzyme electrode fabricated entirely using screen-printing technology is described. The sensor is based on immobilization of lactate oxidase from Pediococcus sp. by printing and subsequent UV irradiation within a polymerizable paste containing different supplements. The sensor optimization is described with respect to the most important features, such as composition and viscosity of the paste, stability, optimum pH and analytical range. The sensor was applied in a flow-through chamber with in a flow injection (FI) system based on dialysis to off-line and on-line bioprocess monitoring of Geotrichum candidum cultivations in complex media. The sensor exhibited good operational stability; 4200 single injections (60 measurements per hour) led to a decrease of only 8.7%, the standard deviation being
Databáze:	OpenAIRE
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