4-Nitrophenol in 4-nitrophenyl phosphate, a substrate for alkaline phosphatase, as measured by paired-ion high-performance liquid chromatography

Autor:	C A Burtis, I W Duncan, P H Culbreth
Rok vydání:	1977
Předmět:	Solvent chemistry.chemical_compound Chromatography chemistry Elution Reagent Biochemistry (medical) Clinical Biochemistry Alkaline phosphatase 4-Nitrophenylphosphatase Substrate (chemistry) Phosphate High-performance liquid chromatography
Zdroj:	Clinical Chemistry. 23:2288-2291
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/23.12.2288
Popis:	We used paired-ion high-performance liquid chromatography to determine the 4-nitrophenol content of 4-nitrophenyl phosphate, a substrate for alkaline phosphatase analysis. This was done on a reversed-phase column with a mobile phase of methanol/water, 45/55 by vol, containing 3 ml of tetrabutylammonium phosphate reagent per 200 ml of solvent. At a flow rate of 1 ml/min, 4-nitrophenol was eluted at 9 min and monitored at 404 nm; 4-nitrophenyl phosphate was eluted at 5 min and could be monitored at 311 nm. Samples of 4-nitrophenyl phosphate obtained from several sources contained 0.3 to 7.8 mole of 4-nitrophenol per mole of 4-nitrophenyl phosphate.
Databáze:	OpenAIRE
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