SEQUENCES OF INTEREST: Homologous Sequences in Steroidogenic Enzymes, Steroid Receptors and a Steroid Binding Protein Suggest a Consensus Steroid-Binding Sequence
| Autor: | James Picado-Leonard, Walter L. Miller |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
chemistry.chemical_classification
Steroid hormone receptor medicine.medical_treatment Cholesterol side-chain cleavage enzyme General Medicine Biology Molecular biology Steroid Amino acid Steroid hormone Endocrinology Biochemistry chemistry Consensus sequence medicine Binding site Molecular Biology Peptide sequence |
| Zdroj: | Molecular Endocrinology. 2:1145-1150 |
| ISSN: | 1944-9917 0888-8809 |
| Popis: | The amino acid sequences of two steroidogenic enzymes, P450c17 (steroid 17α-hydroxylase/17,20 lyse) and P450c21 (steroid 21-hydroxylase), are only 28.9% identical. However, these proteins share a region of 21 amino acids bearing 17 identical residues, which we previously suggested may represent the steroid binding site. We assembled a sequence database of known steroid-binding proteins and searched this with the sequence of this 21 amino acid region. The steroidogenic enzymes, P450c17, P450c21, P450scc (the cholesterol side-chain cleavage enzyme), and P450c11 (steroid 11β18-hydroxylase) share a subregion of 17 amino acids having at least 15 identical residues. Related sequences were identified in a computerized search of the available sequences of steroid hormone receptors and binding proteins. These sequences were invariably found within larger domains previously associated with steroid binding. From these we propose a more general consensus sequence of , where ± refers to any charged amino acid, and 0 r... |
| Databáze: | OpenAIRE |
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