A MITE Insertion in the Promoter Region of Anthocyanidin Synthase from Morus alba L

Autor:	Xiangyun Chen, Changying Liu, Jun Li, Maode Yu, Yaofeng Li, Aichun Zhao, Xiaoqing Liu
Rok vydání:	2018
Předmět:	0106 biological sciences 0301 basic medicine Transposable element chemistry.chemical_classification Reporter gene biology fungi Flavonoid food and beverages Promoter Plant Science biology.organism_classification 01 natural sciences Molecular biology 03 medical and health sciences Leucoanthocyanidins 030104 developmental biology Enzyme chemistry Arabidopsis thaliana Molecular Biology Gene 010606 plant biology & botany
Zdroj:	Plant Molecular Biology Reporter. 36:188-194
ISSN:	1572-9818 0735-9640
Popis:	Anthocyanins play important role in plant protection and were closely involved with the plant evolution. Anthocyanidin synthase (ANS) is a late key enzyme in the flavonoid pathway which can catalyze leucoanthocyanidins to anthocyanidins. By our study, we found a miniature inverted-repeat transposable element (MITE) inserting in the promoter of ANS gene of mulberry. We used strawberry to evaluate the activities of ANS promoters from Morus alba and Morus notabilis with the method of Agrobacterium-mediated transient expression. The expression patterns of different promoters were also analyzed in transgenic lines of Arabidopsis thaliana and in this study, GUS was used as reporter gene. The 564-bp MITE insertion was strongly required for the activities of ANS promoter and it may reprogram the expression profiles of ANS gene in mulberry. Our results suggested that the MITE insertion was probably involved in either domestication or natural selection.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::14193808abb6c07f34c0566d72607e84 https://doi.org/10.1007/s11105-018-1069-z Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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