Isolation, identification and optimization of enhanced production of laccase from Galactomyces geotrichum under solid-state fermentation
| Autor: | Farhang Mizani, Fereshteh Raouf, Khadijeh Pourkhanali, Gholam Khayati |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Laccase biology 010405 organic chemistry Microorganism Substrate (chemistry) Geotrichum General Medicine Galactomyces biology.organism_classification 01 natural sciences Biochemistry 0104 chemical sciences chemistry.chemical_compound chemistry Solid-state fermentation 010608 biotechnology Lignin Fermentation Food science Biotechnology |
| Zdroj: | Preparative Biochemistry & Biotechnology. :1-10 |
| ISSN: | 1532-2297 1082-6068 |
| DOI: | 10.1080/10826068.2020.1848867 |
| Popis: | Laccases are a group of oxidases that catalyze the oxidation of a wide range of electron rich substrates like phenolic compounds, lignin and aromatic amines. They are of interest because of their potential to be used in environmental and industrial applications. In this research, potent laccase producer fungi were screened and isolated from olive mill wastewater (OMW). One of the 23 isolated fungi was identified as Galactomyces geotrichum based on 18S rDNA sequence analysis that detected good laccase activity. Produced laccase had a molecular weight of 55 kDa that was confirmed by zymogram analysis. This is the first report about the optimization of laccase Production by G. geotrichum under solid-state fermentation. The optimization was made by the Taguchi design of experiments (DOE) methodology. An orthogonal array (L25) was designed using Minitab 19 software to study four effective process factors in five levels for laccase production. The optimum condition derived was; moisture content (80%), fermentation time (14 day), CuSO4⋅5H2O as the inducer (300 μM), glucose as a co-substrate (5 g/L). Maximum laccase activity of 52.86 (U/g of dry substrate) was obtained using optimum fermentation condition. This study aimed to better understand the laccase producing microorganisms in OMW and take them to OMW treatment that is rich in phenolic compounds. |
| Databáze: | OpenAIRE |
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