Morpho-physiological and proteomic analysis to identify and characterise the traditional rice genotypes for drought tolerance
Autor: | Nazeem Puthiyaveetil Abdulla, R. Beena, Naresh Babu Prathi, Valsala Poothecty Achuthan, Abida Puthenpeedikal Salim |
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Rok vydání: | 2018 |
Předmět: |
0106 biological sciences
0301 basic medicine education.field_of_study Stomatal conductance Spots Drought tolerance Population food and beverages Wilting Context (language use) Plant Science Biology 01 natural sciences 03 medical and health sciences Horticulture 030104 developmental biology Genotype education Agronomy and Crop Science 010606 plant biology & botany Transpiration |
Zdroj: | Indian Journal of Plant Physiology. 23:785-795 |
ISSN: | 0974-0252 0019-5502 |
Popis: | Rice is the major staple food for one-third of the world’s population. Rice requires high amount of water as compared to other cereal crops. In this context, we screened traditional rice genotypes collected from RARS, Pattambi and Ambalavayal for drought tolerance. Based on the morpho-physiological characters, the plants which showed wilting symptoms were screened according to international standard i.e., IRRI leaf score 7.0. The genotype which showed no wilting symptom even after 90% of the plants were showing wilting was identified as tolerant and the plant which showed early wilting symptom was identified as susceptible genotype. The genotype Chuvanna Modan has taken 25 days and the genotype Parambuvattan has taken only 10 days for wilting. After 90% of plants reached IRRI score 7.0 phenotypic observations i.e., electrical conductivity, measurement of stomatal conductance and transpiration rate using Infra red gas analyser were recorded. Root traits were also measured. The identified tolerant and susceptible genotypes were further subjected to 2D gel electrophoresis to identify and characterize the differentially expressed proteins for drought tolerance. Totally four differentially expressed protein spots were identified in Chuvanna Modan. The differential protein spots were sequenced by Matrix Assisted Laser Desorption/Ionization–Time of Flight Mass Spectrometry (MALDI–TOF/MS). Spot 1 and 2 were identified as ribulose bisphosphate carboxylase small subunit, spot 3 was identified as Protein tyrosine phosphatases and spot 4 was identified as Photosystem II stability/assembly factor HCF136. |
Databáze: | OpenAIRE |
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