| Autor: |
Lois M. Crowe, John H. Crowe, Christopher Z. Womersley, Alan S. Rudolph, Lori Appel, David S. Reid |
| Rok vydání: |
1986 |
| Předmět: |
|
| Zdroj: |
Biochimica et Biophysica Acta (BBA) - Biomembranes. 861:131-140 |
| ISSN: |
0005-2736 |
| DOI: |
10.1016/0005-2736(86)90411-6 |
| Popis: |
The ability of various carbohydrates to prevent leakage of a small, water-soluble marker from unilamellar liposomes during freeze-drying has been investigated and compared with the ability of trehalose to prevent such leakage. Mechanisms of the stabilizing effect of the carbohydrates have also been studied. While the ability of the sugars to prevent lipid mixing during freeze-drying is related to their ability to prevent leakage more sugar is required for prevention of leakage than for prevention of lipid mixing. The mass ratio of carbohydrate:lipid (rather than the molarity of the solution) is the important parameter for prevention of lipid mixing and leakage. Calorimetric results suggest that it is the ability of trehalose to prevent phase separation during drying and phase transitions during rehydration that accounts for the stabilizing effect of this carbohydrate. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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