A New Chemically Deactivated Silica-Based Reverse Phase/Ion Exchange Support

Autor:	Binyamin Feibush
Rok vydání:	1996
Předmět:	Analyte Chromatography Ion exchange Clinical Biochemistry Pharmaceutical Science Protonation Reversed-phase chromatography Biochemistry Analytical Chemistry Silanol chemistry.chemical_compound chemistry Phase (matter) Polar Selectivity
Zdroj:	Journal of Liquid Chromatography & Related Technologies. 19:2315-2328
ISSN:	1520-572X 1082-6076
DOI:	10.1080/10826079608017159
Popis:	A new base-deactivated reverse phase chromatographic medium is described. Basic ligands have been incorporated into the silica matrix to interact with the free silanol groups, to supress their activity. Depending on the mobile phase, the support exhibits hydrophobic and/or ion exchange behavior and is stable between pH 1.5 - 7.5. Basic, acidic, polar, or apolar analytes can be separated with excellent selectivity and peak shape. Different selectivities were observed when the pH of the mobile phase was varied above and below the pKa's of acidic analytes. Good separations were also observed for basic compounds, under chromatographic conditions where the bases were free or protonated, respectively.
Databáze:	OpenAIRE
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