Production of insect resistant potato by genetic transformation with a δ-endotoxin gene from Bacillus thuringiensis var. kurstaki
| Autor: | Mariam B. Sticklen, Ramesh C. Saxena, Jianping Cheng, Mark G. Bolyard |
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| Rok vydání: | 1992 |
| Předmět: |
fungi
Genetic transfer food and beverages Kanamycin Plant Science General Medicine Agrobacterium tumefaciens Biology biology.organism_classification Molecular biology law.invention Murashige and Skoog medium law Bacillus thuringiensis Botany Genetics medicine Agronomy and Crop Science Gene Polymerase chain reaction medicine.drug Southern blot |
| Zdroj: | Plant Science. 81:83-91 |
| ISSN: | 0168-9452 |
| Popis: | Solanum tuberosum L. selection FL1607 was transformed using an Agrobacterium tumefaciens binary vector containing DNA encoding a translational fusion between the Bacillus thuringiensis var. kurstaki (B.t.k.) HD-73 δ-endotoxin and neomycin phosphotransferase II (NPT II). Two hundred forty-three shoots were regenerated from 50 inoculated leaf explants. Putatively transformed shoots were selected based on their ability to root in Murashige and Skoog medium supplemented with 50 mg/l kanamycin. DNA sequences encoding NPT II were detected using the polymerase chain reaction in several plants which formed roots in the selection medium. Southern blot hybridization of total DNA isolated from a greenhouse-grown transformed plant demonstrated integration of the translational fusion into the plant genome. In addition, transcription of the gene fusion in the transgenic potato plant was detected by reverse transcription of mRNA followed by amplification using the polymerase chain reaction (PCR). Insect assays, using tobacco hornworm (Manduca sexta) neonate larvae, indicated a significant reduction of leaf consumption in the transgenic plant when compared to a control potato plant. |
| Databáze: | OpenAIRE |
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