115 CHARACTERIZING NEUTROPHIL PROFILES IN HORSES FOR RHINOCEROS CAPTURE

Autor: M. Vandenplas, R. A. Fayrer-Hosken, M. Kruger, S. Giguere, P. Buss
Rok vydání: 2016
Předmět:
Zdroj: Reproduction, Fertility and Development. 28:187
ISSN: 1031-3613
Popis: Conservation of several African species is becoming essential, and efforts to move threatened animals are causing physiological and reproductive problems. To save these species, a more comprehensive knowledge of their biology and response to stressors is required. Capture stress of rhinoceroses has been quantified (Kruger et al. 2011 Reprod. Fertil. Dev. 23, 181–182) by evaluating leucocyte coping capacity (LCC). LCC is the measurement of the fluorescence of circulating active neutrophils, then expressed as optical density (OD)/1000 neutrophils. The LCC then provides a standardized value between species as we used identical conditions and reagents. To quantify the role of LCC in rhinoceros conservation, it is essential to characterize normal LCC profiles of healthy unstressed rhinoceroses. Horse neutrophils are very similar to rhinoceros neutrophils in their biological activity. The objective of the study was to characterize normal LCC profiles in stallions, geldings, nonpregnant mares, pregnant mares, as well as fillies and colts of various ages as a benchmark for adult and juvenile rhinoceroses. The LCC profiles are shown in Figures 1 to 3. For the colts (days, weeks, and months old) there was little difference in their LCC profile over time. For the fillies (days, weeks, and months old) the LCC response for fillies only days old was significantly (P
Databáze: OpenAIRE