Time-Resolved Fluoroimmunoassay for the Measurement of Androstenone in Porcine Serum and Fat Samples
| Autor: | Timo Lövgren, Riikka Harpio, Mika Tuomola, Heikki Mikola, Pia Knuuttila |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
endocrine system
medicine.medical_specialty Porcine serum Boar taint BOAR Androstenone General Chemistry Biology Biological fluid chemistry.chemical_compound Endocrinology Castration Animal science chemistry Internal medicine medicine General Agricultural and Biological Sciences Quantitative analysis (chemistry) |
| Zdroj: | Journal of Agricultural and Food Chemistry. 45:3529-3534 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/jf9702398 |
| Popis: | A simple competitive time-resolved fluoroimmunoassay was developed for the analysis of the boar taint steroid 5α-androst-16-en-3-one in serum and fat samples. Sensitivities of 30 ng/g and 0.8 ng/mL were obtained for fat and serum measurements, and the assays had mean recoveries of 97% and 103%, respectively. The average coefficients for intra- and interassay variation in fat analysis were 6.0%/8.9%, and in serum measurements 5.4%/8.1%, respectively. Boar fat samples contained androstenone in the range of 130−11570 ng/g (n = 106), and in the 108 kg boar group, 58% of samples exhibited fat androstenone levels above 500 ng/g. The serum androstenone concentrations in boars ranged from 1.5 to 237.3 ng/mL (n = 154), but were almost exclusively below the assay range in barrows (n = 124) and gilts (n = 83). A significant (p < 0.0001) correlation was observed between boar serum and fat androstenone concentrations (r = 0.89, n = 102). A direct serum analysis seems to give a reliable indication of the androstenone l... |
| Databáze: | OpenAIRE |
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