Authentication of Canned Tuna and Bonito by Sequence and Restriction Site Analysis of Polymerase Chain Reaction Products of Mitochondrial DNA
| Autor: | Michal L. Ram, Fadi F. Baidoun, Jeffrey L. Ram |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Genetics
Euthynnus biology Euthynnus affinis Cytochrome b Base pair food and beverages General Chemistry biology.organism_classification Molecular biology Restriction fragment law.invention Restriction site law biology.protein General Agricultural and Biological Sciences Bonito Polymerase chain reaction |
| Zdroj: | Journal of Agricultural and Food Chemistry. 44:2460-2467 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/jf950822t |
| Popis: | Methods of authenticating already canned fish were developed, using polymerase chain reaction (PCR) followed by sequencing and restriction site analysis. The canning process degrades DNA to fewer than 123 base pairs (bp) in length. Therefore, degenerate PCR primers were designed to amplify short ( |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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