Position, orientation, and relative quantum yield ratio determination of fluorescent nanoemitters via combined laser scanning microscopy and polarization measurements
| Autor: | Rafael Jaculbia, Norihiko Hayazawa, Maria Vanessa Balois, Lean Dasallas, Wilson Garcia |
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| Rok vydání: | 2018 |
| Předmět: |
Materials science
Laser scanning Isotropy Quantum yield 02 engineering and technology 021001 nanoscience & nanotechnology Polarization (waves) 01 natural sciences Molecular physics Fluorescence Electronic Optical and Magnetic Materials Dipole Quantum dot 0103 physical sciences Near-field scanning optical microscope 010306 general physics 0210 nano-technology |
| Zdroj: | Optical Materials Express. 8:1290 |
| ISSN: | 2159-3930 |
| Popis: | We present a universal method of determining the position, 3D orientation, and relative quantum yield ratio (RQYR) of fluorescent nanoemitters (ZnS coated CdSe quantum dots) in a glass slide by combining laser scanning microscopy (LSM) and polarization measurements. The quantum dots were located through LSM intensity maps using azimuthal, radial, and linear incident polarizations. LSM imaging was not sufficient to determine the orientation of the quantum dots due to the isotropic absorption dipole moment. The 3D orientation was obtained through polarization measurement. By combining LSM and polarization measurements, the RQYR of a single molecule was evaluated, allowing us to compare the quantum yield of the nanoemitters. |
| Databáze: | OpenAIRE |
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