Expression of Protein Markers of Adipogenesis in Endometriotic Lesions

Autor: N. Yu. Shved, Maria I. Yarmolinskaya, Vladislav S Baranov, Arseniy S. Molotkov, Olga V. Malysheva, N. S. Osinovskaya, O. S. Kopteva, Yu. S. Krylova
Rok vydání: 2020
Předmět:
Zdroj: Cell and Tissue Biology. 14:129-138
ISSN: 1990-5203
1990-519X
Popis: Endometriosis is a widespread disorder in which a tissue morphologically and physiologically similar to endometrium is observed outside the uterine cavity. Earlier, we identified a number of genes that are overexpressed in ectopic endometrium compared to eutopic endometrium. C7, FABP4, ADH1B, and PLA2G2A were the most expressed genes. The difference in their expression level was 200–300 times higher. In this work, we verified the results using real-time PCR and immunohistochemistry and examined the expression of these genes in the peritoneum of patients with endometriosis and healthy women. We have shown that a high level of expression of these genes is due to endometriotic lesions and peritoneal cells rather than eutopic endometrium. The proteins encoded by these genes (FABP4, PLA2G2A, and ADH1B) are known to be adipogenesis markers. Protein C7 is also involved in the differentiation of adipose stem cells. The equally high level of expression of these genes in endometriosis nodules and underlying peritoneum may point to a common origin of these tissues, which supports the metaplastic theory of the origin of endometriosis. In addition, our data can be interpreted as an indication that adipogenic stem cells may play a role in the pathogenesis of endometriosis. Also, our data can be interpreted as an indication of a possible involvement of adipogenic stem cells in the pathogenesis of endometriosis.
Databáze: OpenAIRE
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