Faecal microbial populations of growing kittens fed high- or moderate-protein diets
| Autor: | Carolyn J. Apanavicius, Ingmar S. Middelbos, Brittany M. Vester, Beth L. Dalsing, Dustin C. Lubbs, Kelly S. Swanson |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Litter (animal)
genetic structures General Veterinary biology General Medicine Clostridium perfringens medicine.disease_cause biology.organism_classification Microbiology Animal science Lactobacillus medicine Weaning Animal Science and Zoology sense organs Animal nutrition Feces Temperature gradient gel electrophoresis Bifidobacterium |
| Zdroj: | Archives of Animal Nutrition. 63:254-265 |
| ISSN: | 1477-2817 1745-039X |
| DOI: | 10.1080/17450390902860000 |
| Popis: | The objective of this study was to investigate the intestinal microbiota of growing kittens fed moderate- or high-protein diets using DNA-based qualitative and quantitative techniques. Kittens were weaned to a high-protein (HP; n = 7) or moderate-protein (MP; n = 10) diet at 8 weeks of age. Fresh faecal samples were collected at 8, 12, and 16 weeks of age. DNA was extracted and quantitative PCR used to quantify Bifidobacterium, Lactobacillus, Clostridium perfringens, and Escherichia coli concentrations. Denaturing gradient gel electrophoresis was performed to create a dendrogram and unrooted trees using Bionumerics 5.0 to identify similarity due to litter, age, or diet. Kittens fed HP had lower (p = 0.02) Bifidobacteria and Lactobacillus counts than MP-fed kittens. E. coli was lower (p = 0.02) in HP-fed kittens and tended to be affected by age (p = 0.09). Kittens were clustered by litter at 8 weeks of age, and then clustered by diet at 12 and 16 weeks of age. Our data suggest that faecal microbiota of gro... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |