A Capillary GC Method Using Nitrogen Phosphorus Detection for Determination of Topiramate in Patients with Epilepsy

Autor: V. Prochazkova, Hana Brozmanova, Jana Malakova, Viktor Vorisek, Vladimir Palicka
Rok vydání: 2007
Předmět:
Zdroj: Chromatographia. 66:363-367
ISSN: 1612-1112
0009-5893
Popis: A new capillary gas chromatographic assay with nitrogen phosphorus detection for the determination of topiramate in the serum was developed and validated. The sample procedure included a liquid–liquid extraction of 0.1 mL of alkalized sample with ethyl acetate. The organic solvent was evaporated, and flash methylation of topiramate and internal standard 5-(p-methylphenyl)-5-phenylhydantoin with trimethylanilinium hydroxide (0.07 mol L−1 in methanol) was performed. The structure of derivatization product was confirmed using gas chromatography–mass spectrometry. Validation experiments certified suitability of bioanalytical method in the monitoring of serum concentrations in epileptic patients. The limit of detection was found to be 1.69 μmol L−1. The range of applicability and linearity was established from 4.35 to 69.62 μmol L−1. The accuracy and precision reached acceptable values from 86.15 to 109.5% (recovery) respectively, values were from 2.19 to 11.03% (RSD). No interference was found from endogenous substances or studied antiepileptic drugs.
Databáze: OpenAIRE
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