| Autor: | Joachim Hartmann, Jean-Christophe Cassel, Jochen Klein, Michael Hilgert, Konrad Löffelholz, Hélène Jeltsch |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
0303 health sciences
medicine.medical_specialty Microdialysis Serotonin uptake Hippocampus General Medicine Hippocampal formation Biology Serotonergic Biochemistry 3. Good health Lesion 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Endocrinology Internal medicine medicine Cholinergic medicine.symptom 030217 neurology & neurosurgery Acetylcholine 030304 developmental biology medicine.drug |
| Zdroj: | Neurochemical Research. 28:467-472 |
| ISSN: | 0364-3190 |
| DOI: | 10.1023/a:1022852819018 |
| Popis: | The cholinergic inputs to the rat hippocampus were lesioned by intraseptal injections of 192 IgG-saporin. After 15 days, fetal septal cells were grafted into the hippocampus. Thirteen months later, hippocampal acetylcholine (ACh) release was studied by microdialysis. Lesioning reduced basal ACh release (100%) to 20% of normal, which was compensated for by the graft (71%). Infusion of the serotonin uptake inhibitor citalopram (100 μM) enhanced ACh release to the same extent (% of basal release) in all rat groups. Systemic injection of 8-OH-DPAT (0.5 mg/kg, SC), an agonist of 5-HT1A receptors, caused a smaller ACh release than citalopram. Acetylcholinesterase (AChE) staining and densitometric quantification revealed that the lesion-induced reduction of the AChE-staining density was compensated for by septal grafting. In conclusion, both histochemical and biochemical methods showed that cholinergic hippocampal parameters were drastically impaired by 192 IgG-saporin lesions, but were almost completely restored by septal grafting. The graft responded to intrinsic serotonergic regulation. |
| Databáze: | OpenAIRE |
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