Flow Cytometry Assays in Primary Immunodeficiency Diseases
| Autor: | Nicolas Bensen, Maurice R.G. O'Gorman, Joshua A. Zollett |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.diagnostic_test Hyper-IgM Immunodeficiency Syndrome Lymphocyte Disease Biology medicine.disease In vitro Flow cytometry 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Immune system medicine.anatomical_structure Immunology medicine Primary immunodeficiency Gene 030215 immunology |
| Zdroj: | Flow Cytometry Protocols ISBN: 9781493973446 |
| Popis: | The primary immunodeficiency diseases (PIDs) encompass an extremely large and diverse number of clinical disorders caused by mutations in genes that affect virtually every measurable component of our immune systems. Many of the genetic mutations lead to abnormalities that can be detected in circulating peripheral blood cells of suspected patients by flow cytometry and the appropriate combinations of reagents and in vitro manipulations. The flow cytometry procedures that have been developed to detect abnormalities in peripheral blood cells of primary immunodeficiency patients can barely be covered in an entire book, let alone one chapter. Instead of attempting to cover each disease with a specific assay or test, we review three procedures each covering a global aspect of the observed immune abnormality, i.e., detection of lymphocyte subset abnormalities, lymphocyte "marker" abnormalities, and leukocyte function abnormalities. |
| Databáze: | OpenAIRE |
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