| Autor: |
J. Sire, A.M. Bordes, W. Sly, Y. Surdin, H. De Robichon-Szulmajster |
| Rok vydání: |
1965 |
| Předmět: |
|
| Zdroj: |
Biochimica et Biophysica Acta (BBA) - General Subjects. 107:546-566 |
| ISSN: |
0304-4165 |
| DOI: |
10.1016/0304-4165(65)90199-6 |
| Popis: |
The system for the accumulation of amino acids in Saccharomyces cerevisiae acts as an enzymatic active transport system or amino acid permease. It can concentrate exogenous amino acids up to 1000-fold. All the amino acids studied were concentrated by the amino acid permease, but with widely varying affinties. The D -forms were also concentrated, but with affinities much lower than those for L -forms. A mutant with a 10-fold lower level of amino acid permease has been isolated. The properties of the residual permease of this mutant are the same as those of the parent strain. The character is monogenic and the allele (aap) is recessive. This mutant is resistant to several amino acid analogues. Several observations concernig the study of the repression of biosynthetic enzymes in S. cerevisiae (antagonistic effects of amino acids not structurally related to the exogenous repressor; tempoary maintenance in a repressnted state; efficacity of the system of control by repression) can be explained by the properties of the amino acid permease and of the amino pool in this organism. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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